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Image Search Results
Journal: Cell reports
Article Title: Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence
doi: 10.1016/j.celrep.2018.06.110
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081058 DMEM/F12 Thermo Fisher Cat# 11330–032 DMEM/F12 Phenol red free Thermo Fisher Cat# 11330–021 horse serum Thermo Fisher Cat# 16050–122 cholera toxin Sigma Cat# C8052–2mg epidermal growth factor Peprotech Cat# AF-100–15 Insulin Sigma Cat# I1882–200mg Hydrocortisone Sigma Cat# H0888–1g Critical Commercial Assays Click-iT EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# {"type":"entrez-nucleotide","attrs":{"text":"C10340","term_id":"1535411","term_text":"C10340"}} C10340 Experimental Models: Cell Lines MCF-10A cell line ATCC Cat# CRL-10317, RRID:CVCL_0598 MCF10A H2B-mTurquoise DHB-mVenus Laboratory of Tobias Meyer ( Spencer et al., 2013 ) MCF10A mCitrine-Ki67 This study N/A MCF10A mCitrine-Ki67 H2B-mTurquoise DHB-mCherry This study N/A MCF10A mCitrine-Ki67 H2B-mTurquoise Geminin-mCherry This study N/A MCF7 Ki67-YFP p21-mKate Laboratory of Galit Lahav ( Stewart-Ornstein and Lahav, 2016 ) MCF7 Ki67-YFP p21-mKate H2B-mIFP DHB-mTurquoise2 This study N/A OVCAR-3 cell line ATCC Cat# HTB-161, RRID:CVCL_0465 A375 cell line ATCC Cat# CRL-1619, RRID:CVCL_0132 Oligonucleotides FZR1 DsiRNA IDT Cat# hs.Ri.FZR1.13.2 MYBL2 DsiRNA IDT Cat# hs.Ri.MYBL2.13.2 FOXM1 DsiRNA IDT Cat# hs.Ri.FOXM1.13.1 Negative Control DsiRNA IDT Cat# 51–01-14–04 MKI67 crRNA (AAUGUGGCCCACGAG) IDT N/A CRISPR-Cas9 tracrRNA IDT Cat# 1072532 REAGENT or RESOURCE SOURCE IDENTIFIER Software and
Techniques: Recombinant, Imaging, Negative Control, CRISPR, Software